COCHRAN LABORATORY

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Tufts Seminars TUSK knowledgebase Tufts Directory Search Tufts Directory: Search Tufts:   Department of Physiology, Tufts University School of Medicine 136 Harrison Ave., Boston, Massachusetts, 02111, USA Lab and Office: Jaharis Building, Room 708 Directions: Auto, MBTA, Foot, weather Principal Investigator: Brent Cochran, Ph.D., Professor of Physiology PHONE: (617) 636-0442 (office), (617) 636-6744(lab) FAX: (617) 636-6745 EMAIL: COCHRAN@cochranlab.org Coworkers: Maureen Sherry, Graduate Student Andrew Reeves, Graduate Student Amy Simon, Assistant Prof. of Medicine Former lab members Jay Lee, MD/PhD Student (grad 2002) Dae-Won Kim, Graduate Student (grad 2000) Liza Konnikova, MD/PhD student Matthew Kruger, Research Technician Deepa Bhavsar, Postdoctoral Fellow Maciej Kotecki, Postdoctoral Fellow Joan Abrams Graduate Student(grad 2000) Satoe Takahashi, Research Technician Research Interests: My lab is broadly interested in how growth factors such as PDGF regulate cell growth. For several years we have been working on how the c-fos promoter is regulated. We discovered a growth factor inducible called SIF which turns out to be composed of STAT family transcription factors. We are currently interested in the role of STAT3 in cancer and in cancer stem cells in particular. We have been studying this primarily in the context of the glioblastoma brain cancer. The graphic at the top of the page shows cancer stem cells from a human glioblastoma growing as neurospheres on the left and stained with stem cell and neural markers. Another interest of the lab is the development of an annotated database of genes called MedStract in collaboration with a computational linguistics group at Brandeis. Click for a list of projects ongoing. Useful Info Tufts ejournals list Sequence search of RefSeq mRNA database Annotated list of Biological web resources by BG students Useful microarray links Lab Meeting Rotation Search Medline with PubMed Tufts Signal Transduction Group Meeting Physiology Monday Seminars (invited) Physiology Tuesday Seminars (departmental) Biology Week Online journals Top Ten Misconceptions about Immediate Early Genes (IEG's) Curatools Online sequence analysis sites Signaling Pathway Database Signal transduction knowledge environment Search Medline Search Genbank (Blast) Biologist's Control Panel Biology Workbench TRRD gene regulatory elements Search the OWL unified protein database Pedro's BioMolecular Research Tools Projects Mechanism of activation of Stats by PDGF Regulation of c-fos by the Jak/Stat pathway Cell cycle regulation of growth factor signalling Activation of STATs by oxidative stress Role of Cdc37 in signal transduction and the cell cycle Role of TFII-I in c-fos regulation and signal transduction Development of the annotated gene database called MedStract Selected Publications: Konnikova L, Simeone MC, Kruger MM, Kotecki M, Cochran BH. 2005. Signal transducer and activator of transcription 3 (STAT3) regulates human telomerase reverse transcriptase (hTERT) expression in human cancer and primary cells. Cancer Res. 65(15):6516-20. Abstract Konnikova L, Kotecki M, Kruger MM, Cochran BH. 2003. Knockdown of STAT3 expression by RNAi induces apoptosis in astrocytoma cells. BMC Cancer 2:23. free full text, pdf Simon AR, Takahashi S, Severgnini M, Fanburg BL, Cochran BH. 2002. Role of the JAK-STAT pathway in PDGF-stimulated proliferation of human airway smooth muscle cells. Am J Physiol Lung Cell Mol Physiol Jun;282(6):L1296-304. abstract, full text Kim, D.-W. and B.H. Cochran. 2001. Jak2 activates tfii-i and regulates its interaction with extracellular signal-regulated kinase. Mol Cell Biol. 2001 May 15;21(10):3387-97. abstract, pdf Simon AR, Vikis HG, Stewart S, Fanburg BL, Cochran BH, Guan KL. 2000. Regulation of STAT3 by direct binding to the rac1 GTPase. Science 2000 Oct 6;290(5489):144-7. Abstract, pdf Kim, D.-W. and B.H. Cochran. 2000. ERK binds to TFII-I and regulates its activation of the c-fos promoter. Mol. Cell Biol. 20(4):1140-8. abstract, pdf Kotecki, M., P. S. Reddy, and B. H. Cochran. 1999. Isolation and characterization of a near-haploid human cell line. Exp Cell Res, Nov 1;252(2):273-80. abstract, pdf Grammatikakis, N., Lin, J.-H., Grammatikakis, A., Tsichlis, P. N. and Cochran, B. H. (1999). p50cdc37 acting in concert with Hsp90 is required for Raf-1 function. Mol. Cell Biol., 19, 1661-72. abstract, pdf Kim, D.-W., Cheriyath, V., Roy, A. L. and Cochran, B. H. (1998). TFII-I enhances activation of the c-fos promoter through interactions with upstream elements. Mol. Cell. Biol. 18, 3310-3320.abstract, pdf Simon, A, Rai, U., Fanburg, B. Cochran BH. 1998. Activation of the JAK/STAT pathway by reactive oxygen species. Am. J. of Physiology,44, C1640-C1652. abstract, (Download full text PDF file) Hayes TE, Kitchen AM, Cochran BH. 1987. Inducible binding of a factor to the c-fos regulatory region. Proc. Natl. Acad. Sci. (USA) 84: 1272-1276. Sengupta P, Cochran BH. 1991. MATalpha1 can mediate gene activation by a-mating factor. Genes and Dev. 5: 1924-1934. Meyer, D. J., Campbell, G., Cochran, B. H., Argetsinger, L., Larner, A. C., Finbloom, D. S., Carter-Su, C. and Schwartz, J. 1993. Growth hormone induces a DNA-binding factor related to the interferon-stimulated 91-kDa transcription factor. J. Biol. Chem. 269, 4701-4704. Abstracts Activation of the STAT transcription factor pathway in yeast by v-src. Deepa Bhavsar and Brent H. Cochran. Oncogene Meeting 1995 Physiology Dept.**** Health Science Campus visits since August 7, 2005