Title: Gene Quantification & Real-Time PCR or kinetic RT-PCR v.10.1
Date Reviewed: 06/05/2002
Description: A website that describes in great details all the available PCR and RT-PCR methods for detection and quantification. Each method has a very descriptive and user friendly graphic poster, and movies are also available to demonstrate how each method works. The website also compares and contrasts the methods giving strengths and weaknesses of each one. There are also references and posters available that show physiological applications of each method.
Trouble-shooting suggestions and software to analyze and quantitate your results in also provided. Overall, a great site to either get started using any of these methods, or to learn more about them, or to troubleshoot.
Strengths: Describes, compares and contrasts the different methods available for quantitative RT-PCR/PCR. Great graphical representations and descriptions
Weaknesses: Somewhat redundant in the links given
Comprehensiveness: Very comprehensive, covers most up-to-date techniques, and applications
Timeliness: Updated monthly
Ease of Use: Very user-friendly esp. with the graphics and movies
Responsiveness: Very quick server, no problems navigating
Similar or Related Sites: PCR JUMP Station
Overall Evaluation: ***** 5 stars
Reviewer: Tatyana Vorobyova