My lab is broadly interested in how
growth factors such as PDGF regulate cell growth. For several
years we have been working on how the c-fos
promoter is regulated. We discovered a growth factor inducible
called SIF which turns out to be composed of
STAT family transcription factors. We also
study the yeast pheromone response pathway
which is remarkably analogous to some
aspects of fos regulation. Recent attention has focussed on the role of TFII-I in c-fos regulation. We have also
been interested in the
c-myc gene and have recently found a novel myc interacting protein
using the yeast two-hybrid system. Other interests of the lab include
the development of new genetic strategies for studying mammalian
cells and elucidating the relationship between growth factor mediated signal transduction and the cell cycle
machinery. A new interest of the lab is the development of an annotated database of genes called MedStract in collaboration with a computational linguistics group at Brandeis.
Characterization of the myc interacting protein, Rht
Activation of STATs by oxidative stress
Role of Cdc37 in signal transduction and the cell cycle
Role of TFII-I in c-fos regulation and signal transduction
Development of the annotated gene database called
MedStract
Selected Publications:
Kim, D.-W. and B.H. Cochran. 2000. ERK binds to TFII-I and regulates its activation of thec-fos promoter. Mol. Cell Biol. 20(4):1140-8. abstract, pdf
Kotecki, M., P. S. Reddy, and B. H. Cochran. 1999. Isolation and characterization of a near-haploid human cell line. Exp Cell Res, Nov 1;252(2):273-80.
abstract
Grammatikakis, N., Lin, J.-H., Grammatikakis, A., Tsichlis, P. N. and Cochran, B. H. (1999).
p50cdc37 acting in concert with Hsp90 is required for Raf-1 function. Mol. Cell Biol., 19, 1661-72. abstract, pdf
Kim, D.-W., Cheriyath, V., Roy, A. L. and Cochran, B. H. (1998). TFII-I enhances activation of the c-fos promoter through interactions with upstream elements. Mol. Cell. Biol. 18, 3310-3320.abstract
Simon, A, Rai, U., Fanburg, B.
Cochran BH. 1998. Activation of the JAK/STAT pathway by reactive oxygen species. Am. J. of Physiology,44,
C1640-C1652. abstract, (Download full text PDF file)
Hayes TE, Kitchen AM, Cochran BH. 1987. Inducible binding of a
factor to the c-fos regulatory region. Proc. Natl. Acad. Sci. (USA)
84: 1272-1276.
Sengupta P, Cochran BH. 1991. MATalpha1 can mediate gene
activation by a-mating factor. Genes and Dev. 5: 1924-1934.
Meyer, D. J., Campbell, G., Cochran, B. H., Argetsinger, L.,
Larner, A. C., Finbloom, D. S., Carter-Su, C. and Schwartz, J. 1993.
Growth hormone induces a DNA-binding factor related to the
interferon-stimulated 91-kDa transcription factor. J. Biol. Chem.
269, 4701-4704.
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